Coding

Part:BBa_K3002014:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-13)


Mutant PETase for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 1480
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
    Illegal PstI site found at 1480
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 1480
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 1480
    Illegal NgoMIV site found at 242
    Illegal NgoMIV site found at 269
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Three mutations were inserted: R280A, S238F, and W159H (Joo, S. et al., 2018) (Austin, H. et al, 2018). The natural secretion signal of Ideonella sakaiensis was removed. This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part originates from Ideonella sakaiensis (Yoshida, S., 2016).

References