Coding
Part:BBa_K3002014:Design
Designed by: Dorothée Klein Group: iGEM19_TU_Kaiserslautern (2019-10-13)
Mutant PETase for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 507
Illegal PstI site found at 1480 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 507
Illegal PstI site found at 1480 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 507
Illegal PstI site found at 1480 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 507
Illegal PstI site found at 1480
Illegal NgoMIV site found at 242
Illegal NgoMIV site found at 269 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Three mutations were inserted: R280A, S238F, and W159H (Joo, S. et al., 2018) (Austin, H. et al, 2018). The natural secretion signal of Ideonella sakaiensis was removed. This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.
Source
This part originates from Ideonella sakaiensis (Yoshida, S., 2016).